Well the hive was definitely queenless. As a experiment to compare to my other hive I ordered a Wildflower Meadows VSH Italian mated queen Monday, we got the queen Wednesday and installed her in the JZBZ cage.
The day before we installed, we opened the hive, got rid of the emergency queen cells and added a frame of brood from the other hive along with the nurse bees present. On Wednesday we put the queen cage on that frame of brood. The bees did not attack the cage, most just licked it and fanned their wings so I think they are happy to have a queen around. Next Wednesday will be our inspection to make sure she got out and is laying.
We will then compare the non VSH hive to this one for mite levels.