# Artificial insemination of queen bee problems with laying eggs

**URL:** <https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739>\
**Category:** Bee Breeding\
**Created:** [April 17, 2018, 6:51pm UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739 "2018-04-17T18:51:44Z")\
**Posts on this page:** 6\
**Page:** 1

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**Author:** ![MSD](https://sea2.discourse-cdn.com/flex016/user_avatar/forum.honeyflow.com/msd/32/14782_2.png) [@MSD](https://forum.honeyflow.com/u/MSD)\
**Post date:** [April 17, 2018, 6:51pm UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739/1 "2018-04-17T18:51:44Z")

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i have problem and i hope i will get the answer from you  
some of my queens after i inseminating them. the queens didn’t start laying eggs. i waited more than 40 days without result  
some of them took 5 min Co2 the day before and the others 5 min co2 the next day of the insemination  
i dont know what is the problem with them ?

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**Author:** ![Michael\_Bush](https://sea2.discourse-cdn.com/flex016/user_avatar/forum.honeyflow.com/michael_bush/32/1024_2.png) [@Michael\_Bush](https://forum.honeyflow.com/u/Michael_Bush)\
**Post date:** [April 17, 2018, 7:38pm UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739/2 "2018-04-17T19:38:06Z")

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How long between emergence and insemination?

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**Author:** ![MSD](https://sea2.discourse-cdn.com/flex016/user_avatar/forum.honeyflow.com/msd/32/14782_2.png) [@MSD](https://forum.honeyflow.com/u/MSD)\
**Post date:** [April 18, 2018, 7:49am UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739/3 "2018-04-18T07:49:49Z")

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Usually between 8-9 days old

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**Author:** ![Michael\_Bush](https://sea2.discourse-cdn.com/flex016/user_avatar/forum.honeyflow.com/michael_bush/32/1024_2.png) [@Michael\_Bush](https://forum.honeyflow.com/u/Michael_Bush)\
**Post date:** [April 18, 2018, 12:01pm UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739/4 "2018-04-18T12:01:36Z")

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That sounds about right. I asked because if you wait too long that will result in queens who can’t lay fertilized eggs and if the bees keep up they will remove the drone eggs in worker brood for a while. There are a lot of tricks to II on queens. CO2 of course is one.

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**Author:** ![MSD](https://sea2.discourse-cdn.com/flex016/user_avatar/forum.honeyflow.com/msd/32/14782_2.png) [@MSD](https://forum.honeyflow.com/u/MSD)\
**Post date:** [April 18, 2018, 12:11pm UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739/5 "2018-04-18T12:11:56Z")

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i checked the spermatheca of some of them look perfect full creamed color  
should i give them third Co2 treatment

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**Author:** ![Michael\_Bush](https://sea2.discourse-cdn.com/flex016/user_avatar/forum.honeyflow.com/michael_bush/32/1024_2.png) [@Michael\_Bush](https://forum.honeyflow.com/u/Michael_Bush)\
**Post date:** [April 19, 2018, 1:09pm UTC](https://forum.honeyflow.com/t/artificial-insemination-of-queen-bee-problems-with-laying-eggs/14739/6 "2018-04-19T13:09:31Z")

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I can’t say if it will help, but it probably won’t hurt.