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All life-cycle stages of the wax moth, including eggs, are killed by freezing at the following temperatures and time-lenghs:
−6.7C at 4.5 hours
−12.2C at 3 hours
−15.0C at 2 hours.
It is important to commence timing of this treatment only when the hive material or apiary products have reached the recommended temperature. A number of hobby beekeepers have purchased small freezers and find freezing an excellent means of protecting their combs.
After freezing, the combs should be stored in a moth-proof environment to prevent re-infestation. Sealed, strong plastic garbage bags provide a good insect-proof storage environment.
Combs that have been frozen and then placed in untreated supers for storage are immediately at risk to infestation because the supers may contain wax moth eggs. It is best to treat the super and combs and then place them together in a sealed plastic bag.
The use of cool rooms to store combs and protect them from wax moths has become increasingly popular in the beekeeping industry. A temperature of 4°C will restrict wax moth activity.