I split my hive today to give a friend some frames full of bees and brood. I have two brood boxes and my Honeyflow super. The 2nd brood box looked to be full of honey and no brood. I found that I had so many bees with all three boxes completely full. I thought this was the case and was afraid of the bees swarming. We went and picked up a new queen today. Came back and harvested 21 bottles out of my Honeyflow (2nd time in a month - See picture). I then removed the Honeyflow and the 2nd brood box. I did not pull frames in the 2nd brood box but just simply set aside on a table and looked in from the top. I then went through bottom brood box frame by frame looking for the queen. All kinds of brood with very little honey on most of the 8 frames. Relooked at each frame over and over with no success. It was starting to get late and had to make a decision. Decided to take 5 of the frames in a Nuc box over and set them up at my friends house and installed the new queen leaving the queen in the queen box leaving her to her helpers to eat out the sugar plug.
Question: How do I know if I actually transferred my queen to my friend and I no longer have a queen or not? What can I observe in either of our hives?